ELISA Assay for Human Cross Linked C Telopeptide Of Type II Collagen ''CTXII'' [Competitive Inhibition] 10x 96 wells plate

The ELISA Assay for Human Cross Linked C Telopeptide of Type II Collagen (CTXII) [Competitive Inhibition] is a diagnostic tool used for the quantification of CTXII, a biomarker associated with cartilage degradation, in human serum, plasma, or other biological samples.

Technical Description:

• Target Analyte: CTXII (Cross-linked C Telopeptide of Type II Collagen), a biomarker released during the breakdown of type II collagen in cartilage. It is a fragment of the C-terminal telopeptide region, which is released into the bloodstream when type II collagen is degraded, making it useful for monitoring joint diseases, such as osteoarthritis or rheumatoid arthritis.
• Assay Principle:
  • This is a competitive inhibition ELISA. The assay uses a solid-phase antibody to capture the CTXII peptide from the sample.
  • The competitive inhibition format means that the presence of CTXII in the sample competes with a labeled conjugate of CTXII for binding to the capture antibody. The amount of conjugate that binds to the antibody is inversely proportional to the amount of CTXII in the sample.
  • After washing away unbound components, the remaining bound conjugate is detected using a substrate reaction, which results in a measurable signal.
• Sensitivity: This assay typically has a high sensitivity to detect low concentrations of CTXII, making it suitable for monitoring early stages of cartilage degradation and for evaluating treatments targeting joint health.
• Quantification Range: The assay generally provides a linear detection range from pg/mL to ng/mL, depending on the kit specifications. It is designed to measure CTXII concentrations accurately within this range.
• Detection Method: Most commonly, the assay uses colorimetric detection, where a substrate is catalyzed by an enzyme (usually HRP or alkaline phosphatase) to produce a measurable signal. The signal can be quantified via a spectrophotometer, typically at 450 nm.
• Application:
  • Monitoring joint diseases such as osteoarthritis, rheumatoid arthritis, or chondrolysis.
  • Research into cartilage degradation and regeneration.
  • Assessment of efficacy for treatments aimed at cartilage repair or disease prevention.
• Advantages:
  • High specificity and sensitivity for CTXII.
  • Suitable for clinical and research settings.
  • Easy to perform with minimal equipment and reagents.
• Sample Requirements: Serum, plasma, or other suitable biological fluids. Sample preparation might include simple dilution, and some assays may require serum separation prior to testing.
• Kit Components:
  • Pre-coated microtiter plate with CTXII antibody.
  • Enzyme-labeled detection antibody.
  • Reagents for sample incubation, wash buffers, and substrate for detection.
• Storage and Stability: The reagents and samples must be stored at the recommended temperatures (often 2-8°C for reagents, -20°C for some kits) to maintain stability and accuracy. The shelf life of the kit typically ranges from 6 months to 1 year when stored appropriately.