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Interplay Between Splicing and Small RNAs
Both alternative splicing and small RNAs serve as pivotal post-transcriptional regulatory mechanisms in eukaryotes. Our long-term research investigates the interplay between these two processes, with a focus on their dynamic roles in gene expression regulation in plants. This project aims to bridge knowledge gaps and provide comprehensive insights into RNA-based regulation.

Upcoming Event: Celebrating Groundbreaking Research in Plant Genomics
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Rainbow Calibration Particles in Fluorescence Microscopy
Rainbow calibration particles with 8 peaks are critical for fluorescence microscopy calibration, providing precise emission spectra at eight distinct wavelengths. These particles contain fluorescent dyes embedded in a matrix, allowing them to serve as reference standards for aligning optical filters, detectors, and excitation sources.
In technical terms, they enable the validation of excitation-emission matrices (EEMs), ensuring accurate spectral separation and minimizing signal overlap in multi-channel imaging systems. Calibration particles are particularly effective for quantifying fluorophore intensity, correcting for photobleaching effects, and establishing reproducibility in fluorescence-based assays.
By using these particles, researchers can perform spectral unmixing, which resolves overlapping fluorescence signals from different fluorochromes. This process ensures accuracy in colocalization studies, protein-protein interaction analysis, and fluorescence lifetime imaging (FLIM). They are also critical for maintaining the sensitivity of photomultiplier tubes (PMTs) or CMOS detectors and validating performance in confocal, widefield, and super-resolution microscopy.
Additionally, rainbow calibration particles support the standardization of fluorescence intensity units (e.g., MESF—Molecules of Equivalent Soluble Fluorochrome), ensuring comparability across instruments. They are routinely employed in validating laser stability, spectral registration, and uniform illumination profiles.